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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(2)
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Citations:
(238)
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Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494L
Quantity
500 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (238)
(1)
Structural basis for flagellin-induced NAIP5 activation
Sci Adv
December 6, 2023
Bhaskar Paidimuddala, Jianhao Cao, Liman Zhang
site-directed mutagenesis
was performed with either
Q5 hot start high-fidelity DNA polymerase
-2X master mix (
NEB
, no.
M0494S
) or
KOD hot start DNA polymerase
(
MilliporeSigma
, no. 71086). pET15b-LFn-FliC plasmid was generated
(2)
Differentiated cultures of an immortalized human neural progenitor cell line do not replicate prions despite PrP(C) overexpression
Prion
December 1, 2023
Slota JA, Wang X, Lusansky D, Medina SJ, Booth SA.
50 ng of genomic DNA were amplified with the Q5 Hot Start High-Fidelity 2× PCR Master Mix (cat no.
M0494L
,
NEB
) using primers 5’-TCTTTGTGACTATGTGGACTG-3’ and 5’-TGCCACATGCTTGAGGTTGGTT-3’. The
PCR
products were
(3)
PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina
Nucleus (Austin, Tex.)
December 1, 2023
Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.
pCMV6-AN-mGFP-
PRR14
full-length vectors using Q5 Hot-start High-Fidelity Polymerase 2x Mix (
New England Biolabs
, cat#
M0494S
) and the following primers containing
HindIII
and
RsrII
restriction sites (Forward (
HindIII
): 5’- GCTTCTCAAGCTTGTACCATCCATGGACTTGCCCGG-3’;
(4)
Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblasts
Science Advances
December 1, 2023
Katherine Ilia, Nika Shakiba, Trevor Bingham, Ross D. Jones, Michael M. Kaminski, Eliezer Aravera, Simone Bruno, Sebastian Palacios, Ron Weiss, James J. Collins,
et al.
backbone. We constructed pL0s from
PCR
fragments generated using high-fidelity Q5 polymerase (
NEB
, #
M0494
); we then assembled these fragments using In-Fusion (Takara Bio, #638948). Oligonucleotides for the
(5)
A novel pathway of levodopa metabolism by commensal Bifidobacteria
Scientific Reports
November 6, 2023
M. S. Cirstea, A. Creus-Cuadros, C. Lo, A. C. Yu, A. Serapio-Palacios, S. Neilson, S. Appel-Cresswell, B. B. Finlay
into a strip tube containing a pre-made 25 µL PCR mix (
Q5 Hot Start High-Fidelity 2X Master Mix
,
NEB
M0494L
) and 8F/926R 16S primers (F: AGAGTTTGATCCTGGCTCAG, R: CCGTCAATTCCTTTRAGTTT). The newly streaked plates
(6)
SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Cell
October 26, 2023
Nora Schmidt, Sabina Ganskih, Yuanjie Wei, Alexander Gabel, Sebastian Zielinski, Hasmik Keshishian, Caleb A. Lareau, Liv Zimmermann, Jana Makroczyova, Cadence Pearce,
et al.
Biolabs,N0437A SMARTscribe Reverse Transcriptase,Takara,639538
Q5 Hot Start High-Fidelity 2x Master Mix
,
New England Biolabs
,
M0494L
Deoxynucleotide Mix
,
Agilent Technologies
,
200415
Lipofectamine RNAiMAX,
Life Technologies
,
13778030
Anti-FLAG®
(7)
The Impact of p70S6 Kinase-Dependent Phosphorylation of Gemin2 in UsnRNP Biogenesis
International journal of molecular sciences
October 25, 2023
Esser LM, Li Q, Jüdt M, Kähne T, Stork B, Grimmler M, Wesselborg S, Peter C.
all
cloning
procedures
, the Q5 High-Fidelity 2× Master Mix (New ENGLAND BioLabs, Frankfurt, Germany, #
M0494S
) and
KLD Enzyme Mix
(
New ENGLAND BioLabs
, Frankfurt, Germany, #
M0554S
) were used. For
cloning
of the
(8)
Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination
Microbial Genomics
October 16, 2023
Dinesh Aggarwal, Diana Rajan, Katherine L. Bellis, Emma Betteridge, Joe Brennan, Catarina de Sousa, Julian CARRIAGE Study Team‡, Sharon J. Parkhill, Marcus C. de Goffau, Marcus C. de Goffau,
et al.
(
M0491
,
New England Biolabs
) or with a premixed version using Q5 Hot Start High-Fidelity 2× Mastermix (
M0494
,
New England Biolabs
).
PCR
amplifications were set up either in triplicate (25 μl each), duplicate (40
(9)
tgCRISPRi: efficient gene knock-down using truncated gRNAs and catalytically active Cas9
Nature Communications
September 11, 2023
Ankush Auradkar, Annabel Guichard, Saluja Kaduwal, Marketta Sneider, Ethan Bier
sites were amplified with the help of specific primers and using the Q5 Hot-start master mix (
NEB
#
M0494S
).
PCR
products were purified prior to
Sanger sequencing
. Data were analyzed using an ICE tool by Synthego
(10)
Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
STAR Protoc
August 15, 2023
Miriam Merenciano, Laura Aguilera, Josefa González
Propionic acid
,
Merck
,Cat # 81910-1L Nipagin,Merck,Cat # H5501-500GR Q5 Hot Start High-Fidelity 2× Master Mix,
NEB
,Cat #
M0494S
DreamTaq Green PCR Master Mix (2×),
Thermo Fisher
,Cat #
K1081
,, Critical commercial
assays
,Critical
(11)
Targeting Sex Determination to Suppress Mosquito Populations
Research Square
July 27, 2023
Omar Akbari, Ming Li, Nikolay Kandul, Ruichen Sun, Ting Yang, Elena Dalla Dalla Benetta, Daniel Brogan, igor antoshechkin, Héctor Sánchez Sánchez C, Yin Peng Zhan,
et al.
(Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (
New England Biolabs
, Cat. #
M0494S
). The generated plasmids were transformed into Zymo JM109 chemically competent
E. coli
(
Zymo Research
,
(12)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
bioRxiv
July 27, 2023
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
and the longer amplicon. The in/out PCRs were carried out with Q5 polymerase (
New England BioLabs
,
M0494S
), while the out/out PCRs were carried out with
PrimeSTAR GXL DNA Polymerase
(
Takara Bio
,
R051A
). Seamless
(13)
Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+
eLife
July 19, 2023
Pragnya Chakraborty, Bipan Kumar Deb, Vikas Arige, Thasneem Musthafa, Sundeep Malik, David I Yule, Colin W Taylor, Gaiti Hasan
modifications were carried out using Q5 Hot Start high-fidelity 2 X Master Mix (
New England BioLabs
, Cat#
M0494L
) using the recommendations of the manufacturer. Primers used in this study (details given in Supplementary
(14)
SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stress
bioRxiv
July 17, 2023
Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovic
separate tube. Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (
NEB
#
M0494
) with primers that add the indexed full Illumina adaptor sequences. To isolate barcoded cDNA, the supernatant
(15)
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy
Nature Communications
June 22, 2023
Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer,
et al.
Nextera-compatible adapters (Supplementary Data 6) using Q5® Hot Start High-Fidelity 2× Master Mix (
M0494L
,
NEB
). One microliter of a
1:100
dilution was used for a second
PCR
attaching sample-specific index
(16)
Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production
bioRxiv
June 19, 2023
Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. Kaplan
performed via gibson assemblies. This involved
amplification
with
Q5 high-fidelity DNA polymerase
(
NEB
#
M0494S
, Ipswich, MA, USA),
DpnI
digestion (
NEB
#
R0176S
),
PCR
cleanup (
NEB
#
T1030S
), assembly with the NEBuilder
(17)
Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissues
bioRxiv
June 15, 2023
Allen Yen, Chase Mateusiak, Simona Sarafinovska, Mariam A. Gachechiladze, Juanru Guo, Xuhua Chen, Arnav Moudgil, Alexander J. Cammack, Jessica Hoisington-Lopez, MariaLynn Crosby,
et al.
distribution of barcodes that were packaged. Materials: Q5 Hot Start High-Fidelity Master Mix (
NEB
M0494
) AMPure XP Reagent (Beckman Coulter
A63882
) or Mag-Bind TotalPure
NGS
beads (Omega Biotek M1378-02) NOTE:
(18)
A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networks
Molecular Systems Biology
June 12, 2023
Max A English, Miguel A Alcantar, James J Collins
Standard Buffer,
New England Biolabs
,
M0484L
Q5® Hot Start High‐Fidelity 2X Master Mix
,
New England Biolabs
,
M0494L
BbsI‐HF
,
New England Biolabs
,
R3539L
BsmBI‐v2
,
New England Biolabs
,
R0739L
FseI
,
New England Biolabs
,
R0588L
SbfI‐HF
,
New England Biolabs
(19)
The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
International Journal of Molecular Sciences
May 24, 2023
Jia Ni, Wenjian Song, Nadia Ahmed Ali, Yayi Zhang, Jiani Xing, Kexing Su, Xingxing Sun, Xiaobo Zhao
coding sequence of the 3×FLAG tag was amplified by
PCR
using Q5 Hot Start High-Fidelity 2×Master Mix (
M0494S
,
NEB
, Ipswich, MA, USA). The following thermal condition was used: 98 °C for 3 min, 33 cycles of 98
(20)
The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae
ISME Communications
May 24, 2023
Katherine M. Elston, Laila E. Phillips, Sean P. Leonard, Eleanor Young, Jo-anne C. Holley, Tasneem Ahsanullah, Braydin McReynolds, Nancy A. Moran, Jeffrey E. Barrick
Integrated DNA Technologies and then amplified
PCR
products using either Q5 Hot-Start Master Mix (#
M0494S
,
New England Biolabs
) or KOD XL (#
71087-3
,
Millipore Sigma
) according to manufacturer’s instructions.
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Reviews
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(1)
8/23/2023
Robust DNA polymerase master mix
Pavan Kumar Chodisetti
I work with numerous strain variants of a bacterium that require verification once in a while. Some genes across the strains exhibit variations in both DNA sequence and length. I amplify these genes ...
Read Review
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
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