Q5 Hot Start High-Fidelity DNA Polymerase M0493L from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity DNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0493L
Quantity500 units
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platformbioRxiv : the preprint server for biologySeptember 11, 2023Kipfer E, Hauser D, Lett MJ, Otte F, Urda L, Zhang Y, Lang CMR, Chami M, Mittelholzer C, Klimkait T.between the adjacent fragments. Primers are listed in Table S5. The Q5 High-Fidelity Polymerase kit (Cat# M0493, NEB) was used for amplification (5 μL 5x Q5 reaction buffer, 0.5 μM of each primer, 200 μM dNTP, 0.02

(2) Seq-ing the SINEs of central nervous system tumors in cerebrospinal fluidCell Rep MedAugust 15, 2023Christopher Douville, Samuel Curtis, Mahmoud Summers, Tej D. Azad, Jordina Rincon-Torroella, Yuxuan Wang, Austin Mattox, Bracha Avigdor, Jonathan Dudley, Joshua Materi, et al.supplemented with an additional 0.5 U of Q5 Hot Start High-Fidelity DNA Polymerase (New England BioLabs, M0493L), 1 μl of 10 mM dNTPs (New England BioLabs, N0447L) and 0.4 μl of 25 mM MgCl2 solution (New England BioLabs

(3) Eliminating Malaria Vectors with Precision Guided Sterile MalesbioRxivJuly 26, 2023Andrea L. Smidler, Reema A. Apte, James J. Pai, Martha L. Chow, Sanle Chen, Agastya Mondal, Héctor M. Sánchez C., Igor Antoshechkin, John M. Marshall, Omar S. AkbariNo. / ID: 69504) from pools of 3 adults, PCR amplified (Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L)), and Sanger sequenced for the 7 gRNA expression cassettes. The gRNAdsxF.1 expression cassette was

(4) A confinable female-lethal population suppression system in the malaria vector, Anopheles gambiaeScience AdvancesJuly 7, 2023Andrea L. Smidler, James J. Pai, Reema A. Apte, Héctor M. Sánchez C., Rodrigo M. Corder, Eileen Jeffrey Gutiérrez, Neha Thakre, Igor Antoshechkin, John M. Marshall, Omar S. Akbari20 μl of PCR reaction using Q5 HotStart DNA polymerase [New England Biolabs (NEB), catalog no./ID: M0493L] and primers 1154A.S5 and 1154A.S7 amplifying genomic fle sequences. The resulting product was run on

(5) A rapid cell-free expression and screening platform for antibody discoveryNature communicationsJuly 3, 2023Hunt AC, Vögeli B, Hassan AO, Guerrero L, Kightlinger W, Yoesep DJ, Krüger A, DeWinter M, Diamond MS, Karim AS, et al.gblocks from IDT. These components were amplified using PCR using Q5 Hot Start DNA polymerase (NEB, M0493L) following manufacturer instructions. Amplified DNA was purified using the DNA Clean and Concentrate

(6) Multiple Clonostachys rosea UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with Fusarium graminearumJournal of FungiJuly 2, 2023Kelly A. Robinson, Antony D. St-Jacques, Sam W. Shields, Amanda Sproule, Zerihun A. Demissie, David P. Overy, Michele C. LoewenSupplemental Table S2 tab; Eurofins Genomics) and the Q5® Hot Start High-Fidelity DNA Polymerase enzyme (NEB, M0493), following the thermocycling conditions specified in the manual, with the annealing temperature at

(7) Combinatorial selective ER-phagy remodels the ER during neurogenesisbioRxiv : the preprint server for biologyJune 26, 2023Hoyer MJ, Smith IR, Paoli JC, Jiang Y, Paulo JA, Harper JW.Reagent Set Thermo Scientific (A44520); Q5 Hot Start High-Fidelity DNA Polymerase New England BioLabs (M0493); Gateway LR Clonase II Enzyme Mix Thermo (11791020); NEBuilder HiFi DNA Assembly Master Mix (E2621s);

(8) Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patientsCell Reports MedicineJune 20, 2023Joann Diray-Arce, Slim Fourati, Naresh Doni Jayavelu, Ravi Patel, Cole Maguire, Ana C. Chang, Ravi Dandekar, Jingjing Qi, Brian H. Lee, Patrick van Zalm, et al.genome was then amplified with Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs, cat. M0493) using two sets of custom-designed tiling primers generating overlapping amplicons of ∼1.5 and 2 kb.

(9) Elucidation of the binding mode of organic polysulfides on the human TRPA1 receptorFrontiers in PhysiologyJune 7, 2023Balázs Nemes, Szabolcs László, Balázs Zoltán Zsidó, Csaba Hetényi, Adam Feher, Ferenc Papp, Zoltan Varga, Éva Szőke, Zoltán Sándor, Erika Pintér(Analytik Jena 846-2-070-2xx) PCR machine and Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs M0493) were used for all PCR reactions, following the manufacturer’s manuals. All primers were designed by

(10) Ubiquitin-independent proteasomal degradation driven by C-degron pathwaysMolecular CellJune 1, 2023Yaara Makaros, Anat Raiff, Richard T. Timms, Ajay R. Wagh, Mor Israel Gueta, Aizat Bekturova, Julia Guez-Haddad, Sagie Brodsky, Yarden Opatowsky, Michael H. Glickman, et al.assays,Critical commercial assays ,, Q5® Hot Start High-Fidelity DNA Polymerase,New England Biolabs,Cat# M0493S QIAEX II Gel Extraction Kit,QIAGEN,Cat# 20051 PCR purification kit,QIAGEN,Cat# 28106 Gentra Puregene

(11) Spinal muscular atrophy-like phenotype in a mouse model of acid ceramidase deficiencyCommunications biologyMay 25, 2023Nagree MS, Rybova J, Kleynerman A, Ahrenhoerster CJ, Saville JT, Xu T, Bachochin M, McKillop WM, Lawlor MW, Pshezhetsky AV, et al.screened for deletion of NeoR (P361R-SMA) by conventional PCR using Q5 polymerase (New England Biolabs M0493S) followed by Sanger sequencing (Retrogen) of the amplicons using the following primers: 5ʹ-GGTAAGAGAGACAACATAGGTGC-3ʹ

(12) Characterizing and utilizing oxygen-dependent promoters for efficient dynamic metabolic engineeringMetabolic EngineeringMay 1, 2023Julian Wichmann, Gerrich Behrendt, Simon Boecker, Steffen Klamt#C2987U). DNA fragments were amplified by PCR using Q5 Hot Start High Fidelity DNA polymerase (NEB, #M0493L) according to manufacturer's instructions. The Zymo-Parts toolkit developed previously in our group

(13) Svep1 is a binding ligand of Tie1 and affects specific aspects of facial lymphatic development in a Vegfc-independent mannereLifeApril 25, 2023Melina Hußmann, Dörte Schulte, Sarah Weischer, Claudia Carlantoni, Hiroyuki Nakajima, Naoki Mochizuki, Didier Y R Stainier, Thomas Zobel, Manuel Koch, Stefan Schulte-MerkerGmbH,F7524, "Chemical compound, drug",Q5 Hot Start High-Fidelity DNA Polymerase,New England Biolabs GmbH,M0493, "Chemical compound, drug","DMEM/F-12, GlutaMAX Supplement",Thermo Fisher Scientific,10565018, "Chemical

(14) Bacterial genome engineering using CRISPR RNA-guided transposasesbioRxivMarch 25, 2023Diego R. Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris Wang, Samuel H. Sternberg)Wizard Genomic DNA Purification Kit (Promega cat. no. A1125) PCR and qPCR Q5 Hot Start High-Fidelity DNA Polymerase (NEB, cat. no. M0493S/L)dNTP mix, 10 mM (NEB, cat. no. N0447S/L)Q5 reaction buffer, 5X (included with enzyme, or purchased

(15) A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in CarcinomasCancer discoveryMarch 1, 2023Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer SM, Shim BH, Dumont N, et al.PCR reaction was performed using Q5 High-Fidelity DNA Polymerase (New England BioLabs, catalog no. M0493L), and the thermocycling condition was 98°C for 30 seconds, followed by 35 cycles of 98°C for 10 seconds,

(16) Streamlined high-throughput cloning protocol to generate arrayed mutant librariesSTAR ProtocolsMarch 1, 2023Kerry T. Sun, Tark S. Patel, Justin Kim, Helen S.H. Tang, Ghazaleh Eskandari-Sedighi, Haresh Sureshkumar, Dean Schieve, S.A. Mok,Thermo Fisher,Cat#10297018 Glycerol,Sigma-Aldrich,CAS 56-81-5 Q5 Hot Start DNA polymerase,New England Biolabs (NEB),Cat#M0493L ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Magjet Plasmid DNA Kit

(17) Programmable RNA detection with CRISPR-Cas12aResearch SquareFebruary 17, 2023Santosh R. Rananaware, Santosh Rananaware, Grace M. Shoemaker, Swapnil S. Shoemaker, Luke Samuel W. Sandoval, Luke Samuel Sandoval, Nicolas C. Macaluso, Nicolas Macaluso, Piyush K. Nguyenthen was PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs, Catalog #M0493S), and subcloned into a bacterial expression vector (Addgene plasmid #29656, a gift from Scott Gradia).

(18) Programmable RNA detection with CRISPR-Cas12abioRxivFebruary 2, 2023Santosh R. Rananaware, Emma K. Vesco, Grace M. Shoemaker, Swapnil S. Anekar, Luke Samuel W. Sandoval, Katelyn S. Meister, Nicolas C. Macaluso, Long T. Nguyen, Piyush K. Jainthen was PCR amplified using Q5 Hot Start high fidelity DNA polymerase (New England Biolabs, Catalog #M0493S), and subcloned into a bacterial expression vector (Addgene plasmid #29656, a gift from Scott Gradia).

(19) Quantifying the local adaptive landscape of a nascent bacterial communityNature CommunicationsJanuary 16, 2023Joao A. Ascensao, Kelly M. Wetmore, Benjamin H. Good, Adam P. Arkin, Oskar Hallatschekng/μL. DNA barcodes were amplified from gDNA samples via PCR with Q5 Hot Start Polymerase (NEB, cat. no. M0493S); 50 ul reactions were composed of 5 μL PCR primers, 5 μL gDNA, 10 μL 5× buffer, 10 μL GC enhancer,

(20) A central role for regulated protein stability in the control of TFE3 and MITF by nutrientsMolecular cellJanuary 5, 2023Nardone C, Palanski BA, Scott DC, Timms RT, Barber KW, Gu X, Mao A, Leng Y, Watson EV, Schulman BA, et al.barcodes at the 3’ end of each ORF were amplified by PCR (NEB, Q5 Hot Start High-Fidelity DNA Polymerase, M0493L). Following a second round of PCR to add the Illumina P5 and P7 adaptor sequences, samples were pooled

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4/9/2015Hi Fidelity Polymerase Benjamin GerovacI used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. Upon sequencing, this polymerase gave a PCR product of the ... Read Review

Q5 Hot Start High-Fidelity DNA Polymerase

Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs

New England Biolabs

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Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs

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