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PCR Master Mixes (High Fidelity)
Q5 Hot Start High-Fidelity 2X Master Mix
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Q5 Hot Start High-Fidelity 2X Master Mix
Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
(3)
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Citations:
(231)
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Supplier Page from
New England Biolabs for
Q5 Hot Start High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 Hot Start High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0494S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (231)
(1)
Differentiated cultures of an immortalized human neural progenitor cell line do not replicate prions despite PrPC overexpression
Prion
December 31, 2023
Jessy A. Slota, Xinzhu Wang, Diana Lusansky, Sarah J. Medina, Stephanie A. Booth
50 ng of genomic DNA were amplified with the Q5 Hot Start High-Fidelity 2× PCR Master Mix (cat no.
M0494L
,
NEB
) using primers 5’-TCTTTGTGACTATGTGGACTG-3’ and 5’-TGCCACATGCTTGAGGTTGGTT-3’. The
PCR
products were
(2)
PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina
Nucleus (Austin, Tex.)
December 1, 2023
Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.
pCMV6-AN-mGFP-
PRR14
full-length vectors using Q5 Hot-start High-Fidelity Polymerase 2x Mix (
New England Biolabs
, cat#
M0494S
) and the following primers containing
HindIII
and
RsrII
restriction sites (Forward (
HindIII
): 5’- GCTTCTCAAGCTTGTACCATCCATGGACTTGCCCGG-3’;
(3)
Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations
STAR Protoc
August 15, 2023
Miriam Merenciano, Laura Aguilera, Josefa González
Propionic acid
,
Merck
,Cat # 81910-1L Nipagin,Merck,Cat # H5501-500GR Q5 Hot Start High-Fidelity 2× Master Mix,
NEB
,Cat #
M0494S
DreamTaq Green PCR Master Mix (2×),
Thermo Fisher
,Cat #
K1081
,, Critical commercial
assays
,Critical
(4)
Targeting Sex Determination to Suppress Mosquito Populations
Research Square
July 27, 2023
Omar Akbari, Ming Li, Nikolay Kandul, Ruichen Sun, Ting Yang, Elena Dalla Dalla Benetta, Daniel Brogan, igor antoshechkin, Héctor Sánchez Sánchez C, Yin Peng Zhan,
et al.
(Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (
New England Biolabs
, Cat. #
M0494S
). The generated plasmids were transformed into Zymo JM109 chemically competent
E. coli
(
Zymo Research
,
(5)
The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiation
bioRxiv
July 27, 2023
Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manu
and the longer amplicon. The in/out PCRs were carried out with Q5 polymerase (
New England BioLabs
,
M0494S
), while the out/out PCRs were carried out with
PrimeSTAR GXL DNA Polymerase
(
Takara Bio
,
R051A
). Seamless
(6)
Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+
eLife
July 19, 2023
Pragnya Chakraborty, Bipan Kumar Deb, Vikas Arige, Thasneem Musthafa, Sundeep Malik, David I Yule, Colin W Taylor, Gaiti Hasan
modifications were carried out using Q5 Hot Start high-fidelity 2 X Master Mix (
New England BioLabs
, Cat#
M0494L
) using the recommendations of the manufacturer. Primers used in this study (details given in Supplementary
(7)
SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stress
bioRxiv
July 17, 2023
Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovic
separate tube. Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (
NEB
#
M0494
) with primers that add the indexed full Illumina adaptor sequences. To isolate barcoded cDNA, the supernatant
(8)
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathy
Nature Communications
June 22, 2023
Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer,
et al.
Nextera-compatible adapters (Supplementary Data 6) using Q5® Hot Start High-Fidelity 2× Master Mix (
M0494L
,
NEB
). One microliter of a
1:100
dilution was used for a second
PCR
attaching sample-specific index
(9)
Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production
bioRxiv
June 19, 2023
Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. Kaplan
performed via gibson assemblies. This involved
amplification
with
Q5 high-fidelity DNA polymerase
(
NEB
#
M0494S
, Ipswich, MA, USA),
DpnI
digestion (
NEB
#
R0176S
),
PCR
cleanup (
NEB
#
T1030S
), assembly with the NEBuilder
(10)
Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissues
bioRxiv
June 15, 2023
Allen Yen, Chase Mateusiak, Simona Sarafinovska, Mariam A. Gachechiladze, Juanru Guo, Xuhua Chen, Arnav Moudgil, Alexander J. Cammack, Jessica Hoisington-Lopez, MariaLynn Crosby,
et al.
distribution of barcodes that were packaged. Materials: Q5 Hot Start High-Fidelity Master Mix (
NEB
M0494
) AMPure XP Reagent (Beckman Coulter
A63882
) or Mag-Bind TotalPure
NGS
beads (Omega Biotek M1378-02) NOTE:
(11)
A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networks
Molecular Systems Biology
June 12, 2023
Max A English, Miguel A Alcantar, James J Collins
Standard Buffer,
New England Biolabs
,
M0484L
Q5® Hot Start High‐Fidelity 2X Master Mix
,
New England Biolabs
,
M0494L
BbsI‐HF
,
New England Biolabs
,
R3539L
BsmBI‐v2
,
New England Biolabs
,
R0739L
FseI
,
New England Biolabs
,
R0588L
SbfI‐HF
,
New England Biolabs
(12)
The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in Chloroplasts
International Journal of Molecular Sciences
May 24, 2023
Jia Ni, Wenjian Song, Nadia Ahmed Ali, Yayi Zhang, Jiani Xing, Kexing Su, Xingxing Sun, Xiaobo Zhao
coding sequence of the 3×FLAG tag was amplified by
PCR
using Q5 Hot Start High-Fidelity 2×Master Mix (
M0494S
,
NEB
, Ipswich, MA, USA). The following thermal condition was used: 98 °C for 3 min, 33 cycles of 98
(13)
The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae
ISME Communications
May 24, 2023
Katherine M. Elston, Laila E. Phillips, Sean P. Leonard, Eleanor Young, Jo-anne C. Holley, Tasneem Ahsanullah, Braydin McReynolds, Nancy A. Moran, Jeffrey E. Barrick
Integrated DNA Technologies and then amplified
PCR
products using either Q5 Hot-Start Master Mix (#
M0494S
,
New England Biolabs
) or KOD XL (#
71087-3
,
Millipore Sigma
) according to manufacturer’s instructions.
(14)
A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regions
Nucleic Acids Research
May 22, 2023
Chen Pan, Ran Li, Liyan Shui, Zhengyun Xiao, Yali Wang, Jing Zhu, Chao Wu, Long Zhang, Junling Jia, Min Zheng
designed via NCBI Primer BLAST, and then
PCR
was conducted with Q5 Hot Start High-Fidelity 2× Mix (
NEB
,
M0494
) (Supplementary Table S11). The
PCR product
was purified via a QIAquick PCR Clean-up Kit and was constructed
(15)
HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcription
Nucleic Acids Research
May 22, 2023
Rui Tian, Zheying Huang, Lifang Li, Jingping Yuan, Qinghua Zhang, Lirong Meng, Bin Lang, Yongfeng Hong, Chaoyue Zhong, Xun Tian,
et al.
polymerase chain reaction (PCR
) was conducted using the
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
,
M0494S
). The primers are listed in Supplementary Table S3.
RT–qPCR
Total RNA was extracted by RNA-easy Isolation
(16)
High-resolution spatial multi-omics reveals cell-type specific nuclear compartments
bioRxiv
May 12, 2023
Yodai Takei, Yujing Yang, Jonathan White, Jina Yun, Meera Prasad, Lincoln J Ombelets, Simone Schindler, Long Cai
from 13 different oligo array pools. Briefly, we performed
PCR
with Q5 Hot Start High-Fidelity (
NEB
M0494S
), column
purification
with QIAquick PCR Purification Kit (
Qiagen
28104
),
in vitro transcription
(
NEB
(17)
DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavage
Nucleic Acids Research
May 8, 2023
Juan Pan, Megumu Mabuchi, Gregory Brett Robb
Monarch® Total RNA Miniprep Kit
(#
T2010S
), Monarch®
PCR
& DNA Cleanup Kit (#
T1030L
), Q5® Hot Start High-Fidelity 2× Master Mix (#
M0494L
),
EcoRI-HF
(#
R3101S
), rSAP (#
M0371S
),
T4 DNA ligase
(#
M0202S
),
NEB
® 10-beta Competent
Escherichia coli
(18)
Improved cytosine base editors generated from TadA variants
Nature Biotechnology
May 1, 2023
Dieter K. Lam, Patricia R. Feliciano, Amena Arif, Tanggis Bohnuud, Thomas P. Fernandez, Jason M. Gehrke, Phil Grayson, Kin D. Lee, Manuel A. Ortega, Courtney Sawyer,
et al.
Multiplex PCR Master Mix (
Thermo Fisher Scientific
,
F564L
),
Q5 Hot Start High-Fidelity 2X Master Mix
(
NEB
,
M0494L
), Mach T1 competent cells (
Thermo Fisher Scientific
,
C8681201
) and ZymoPURE II Plasmid
Midiprep
kits
(19)
Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+
Nature Methods
May 1, 2023
Roger S. Zou, Yang Liu, Oscar E. Reyes Gaido, Maximilian F. Konig, Brian J. Mog, Leo L. Shen, Franklin Aviles-Vazquez, Alberto Marin-Gonzalez, Taekjip Ha
samples were amplified with
PCR
using Q5 HotStart High-Fidelity 2X Master Mix (
New England BioLabs
,
M0494
). Primer pairs for all sequences are listed in Supplementary Table 3. For example, the primer set for
(20)
Late Embryogenesis Abundant Proteins Contribute to the Resistance of Toxoplasma gondii Oocysts against Environmental Stresses
mBio
April 25, 2023
David Arranz-Solís, David Warschkau, Benedikt T. Fabian, Frank Seeber, Jeroen P. J. Saeij
conducted by using either
Q5 Hot Start High-Fidelity DNA polymerase
(
New England Biolabs
, cat. no.
M0494S
) for
cloning
purposes, or MangoMix (Meridian Bioscience ref. BIO-25034) for diagnostic/
screening
purposes.
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Reviews
Write a Review
(1)
10/6/2023
Convenient PCR Master Mix From New England Biolabs
Anh Nguyen
I used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)
3/31/2023
Good PCR Master Mix
YONG M
Try this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)
1/2/2020
DNA Insert Amplification for Cloning
Dr. Thippeswamy Sannasiddappa
Highly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.
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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs
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