APC Annexin V 640920 from BioLegend

Product Specs
ItemAPC Annexin V
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640920
Quantity100 tests
ApplicationsFlow Cytometry (FC)
Conjugate/TagAPC
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapyOncoimmunologyDecember 21, 2023Jiqi Shan, Wei Jing, Yu Ping, Chunyi Shen, Dong Han, Fengsen Liu, Yaqing Liu, Congcong Li, Yi Zhang10:1 in vitro. After 24 h, B16 tumor cells were collected and stained with Annexin V (BioLegend, cat. #640920) and 7-AAD Viability Staining Solution (BioLegend, cat. #420404). Flow cytometry was used to analyze

(2) End-binding protein 1 regulates the metabolic fate of CD4+ T lymphoblasts and Jurkat T cells and the organization of the mitochondrial networkFrontiers in ImmunologyJuly 13, 2023Álvaro Gómez-Morón, Silvia Requena, Clara Pertusa, Marta Lozano-Prieto, Diego Calzada-Fraile, Camila Scagnetti, Inés Sánchez-García, Ana Adela Calero-García, Manuel Izquierdo, Noa B. Martín-Cófreces(Tonbo biosciences) in 100 µl PBS during 30 min at 4°C and with 2 µl APC-conjugated annexin V (No. 640920 Biolegend) in 100 µl Annexin V Binding Buffer (No. 422201, Biolegend) for 15 min at RT, following the

(3) Role of mitochondria in the myopathy of juvenile dermatomyositis and implications for skeletal muscle calcinosisJournal of AutoimmunityJuly 1, 2023Bhargavi Duvvuri, Lauren M. Pachman, Payton Hermanson, Ting Wang, Richard Moore, Dennis Ding-Hwa Ding-Hwa Wang, Aaron Long, Gabrielle A. Morgan, Stephen Doty, Rong Tian, et al.analysis (flow cytometry) Viability analysis was done using APC-conjugated Annexin V (Biolegend Cat. No. 640920 and propidium iodide (Miltenyi Biotec Cat. No. 130–093-233). Briefly, adhered RH30 cells following trypsinization

(4) Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustionMolecular cellApril 20, 2023Battistello E, Hixon KA, Comstock DE, Collings CK, Chen X, Rodriguez Hernaez J, Lee S, Cervantes KS, Hinkley MM, Ntatsoulis K, et al.IFN-γ (BioLegend, Cat# 505825). Annexin staining was performed with APC Annexin V (BioLegend, Cat# 640920). Samples were analyzed using a Fortessa cytometer. Chromatin-focused CRISPR screen Library design For

(5) Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant ActivityInternational Journal of Molecular SciencesApril 12, 2023Jens Van Bael, Aline Vandenbulcke, Abdelhakim Ahmed-Belkacem, Jean-François Guichou, Jean-Michel Pawlotsky, Jelle Samyn, Arjan D. Barendrecht, Coen Maas, Simon F. De Meyer, Karen Vanhoorelbeke, et al.(HEPES)-buffered saline (pH 7.3) in the presence or absence of 2.5 mM CaCl2 and AnnexinV (APC; cat. n° 640920, Biolegend, San Diego, CA, USA). Anti-CD41 (BV421; Biolegend, San Diego, CA, USA) or anti-CD42b (Super

(6) Expansion of circulating stem-like CD8(+) T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in miceNature communicationsApril 12, 2023Onyshchenko K, Luo R, Guffart E, Gaedicke S, Grosu AL, Firat E, Niedermann G.(eBiosciences, 88-8824-00) according to the manufacturer’s instructions. The APC Annexin V/PI Kit (BioLegend, 640920) was used to determine the proportion of apoptotic cells. Data were acquired using a CytoFlex S Flow

(7) Niche stiffness sustains cancer stemness via TAZ and NANOG phase separationNature communicationsJanuary 16, 2023Liu X, Ye Y, Zhu L, Xiao X, Zhou B, Gu Y, Si H, Liang H, Liu M, Li J, et al.incubated with 100 μL binding buffer (Biolegend) containing 5 μL APC-conjugated Annexin V (1:20, cat# 640920, Biolegend) for 15 min at room temperature. After incubation, the cells were washed and resuspended

(8) Phosphoproteomic profiling of T cell acute lymphoblastic leukemia reveals targetable kinases and combination treatment strategiesNature CommunicationsDecember 1, 2022Valentina Cordo’ et al.staining of apoptotic cells, 200,000 cells were stained with Annexin V-APC antibody (Biolegend cat# 640920) diluted 1:20 in Annexin V-binding buffer (Invitrogen cat# V13246) for 15 min at room temperature (RT)

(9) Inhibition of SRPK1, a key splicing regulator, exhibits antitumor and chemotherapeutic-sensitizing effects on extranodal NK/T-cell lymphoma cellsBMC cancerOctober 27, 2022He C, Liu B, Wang HY, Wu L, Zhao G, Huang C, Liu Y, Shan B, Liu L.resistance. Flow cytometry assay Annexin V (420404, Biolegend, USA) and 7-amino-actinomycin D (7-AAD, 640920, Biolegend, USA) double staining was performed to detect cell apoptosis. Cells were analyzed using the

(10) G6PD promotes cell proliferation and dexamethasone resistance in multiple myeloma via increasing anti-oxidant production and activating Wnt/β-catenin pathwayExperimental hematology & oncologyOctober 21, 2022Li R, Ke M, Qi M, Han Z, Cao Y, Deng Z, Qian J, Yang Y, Gu C.Subsequently, cell pellets were resuspended in 100 μL 1 × Binding buffer, to which 5 μL Annexin V-APC (#640920, Biolegend, Beijing, China) and 5 μL PI (50 μg/mL, #25535-16-4, Solarbio Life Science, Beijing, China)

(11) Polyethylenimine, an Autophagy-Inducing Platinum-Carbene-Based Drug Carrier with Potent Toxicity towards Glioblastoma Cancer Stem CellsCancers (Basel)October 15, 2022Conor McCartin, Candice Dussouillez, ChloÃ© Bernhard, Eric Mathieu, Juliette Blumberger, Monique Dontenwill, Christel Herold-Mende, Ahmed Idbaih, Philippe Lavalle, StÃ©phane Bellemin-Laponnaz, et al.140 mM NaCl, 2.5 mM CaCl2, pH 7.3–7.4). Triplicates were pooled and stained with APC-conjugated AnV (#640920, BioLegend, Amsterdam, Netherlands) diluted 1/100 with prepared AnV binding buffer which was incubated

(12) Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasionJournal for ImmunoTherapy of CancerSeptember 23, 2022Yijie Ren et al.(St Louis, MI). The apoptosis analysis was performed using APC Annexin V Apoptosis Detection Kit (#640920) with the Aqua Live/Dead (#423101) from BioLegend (San Diego, California, USA). Glycolysis analysis For

(13) A Planar Culture Model of Human Absorptive Enterocytes Reveals Metformin Increases Fatty Acid Oxidation and ExportCellular and Molecular Gastroenterology and HepatologyApril 28, 2022Ismael Gomez-Martinez, R. Jarrett Bliton, Keith A. Breau, Michael J. Czerwinski, Ian A. Williamson, Jia Wen, John F. Rawls, Scott T. Magnessanti-human hashtag 6 antibody,"BioLegend (San Diego, CA)",394641 Annexin V-APC,"BioLegend (San Diego, CA)",640920 RNAqueous-Micro Total RNA Isolation Kit,"Thermo Fisher (Waltham, MA)",AM1931 "Chromium Next GE M Single-Cell

(14) Oncolytic Herpes Simplex Virus Type 1 Induces Immunogenic Cell Death Resulting in Maturation of BDCA-1(+) Myeloid Dendritic CellsInternational journal of molecular sciencesApril 27, 2022Kalus P, De Munck J, Vanbellingen S, Carreer L, Laeremans T, Broos K, Dufait I, Schwarze JK, Van Riet I, Neyns B, et al.were resuspended in 100 µL composed of 2 µL AnnexinV-APC (1/50; BioLegend, Amsterdam, Netherlands; 640920), 3.3 µL DAPI solution (0.03 µg/mL) and 94.7 µL 1X Annexin V Binding Buffer (BD Biosciences, 55645).

(15) Ca²⁺-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repairNature communicationsApril 6, 2022Niekamp P, Scharte F, Sokoya T, Vittadello L, Kim Y, Deng Y, Südhoff E, Hilderink A, Imlau M, Clarke CJ, et al.µM ionomycin or 0.1% (v/v) DMSO for 10 min at 37 °C in 5% CO2. Next, APC-Annexin V (Biolegend, no. 640920; 5 µl in 100 µl Binding Buffer) and propidium iodide (5 µg/ml; Sigma-Aldrich, P4170) were added and

(16) Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liverScience advancesMarch 18, 2022Lee Y, DiMaulo-Milk E, Leslie J, Ding L.(1 μg/ml). Annexin V staining was performed using Annexin V Apoptosis Detection Kit APC (BioLegend, 640920) per the manufacturer’s instructions. Intracellular staining For intracellular staining, cells were

(17) Integrated Multiomic Profiling Identifies the Epigenetic Regulator PRC2 as a Therapeutic Target to Counteract Leukemia Immune Escape and RelapseCancer discoveryMarch 7, 2022Gambacorta V, Beretta S, Ciccimarra M, Zito L, Giannetti K, Andrisani A, Gnani D, Zanotti L, Oliveira G, Carrabba MG, et al.V–positive cells at the end of the assay within the population of blasts (BioLegend, APC Annexin V; cat. #640920, lot #B334668). Untreated target cells served as a control to detect the basal proportion of apoptotic

(18) Kupffer Cells and Blood Monocytes Orchestrate the Clearance of Iron-Carbohydrate Nanoparticles from SerumInternational journal of molecular sciencesFebruary 28, 2022Arsiwala T, Vogt AS, Barton AE, Manolova V, Funk F, Flühmann B, Bachmann MF.Franklin Lakes, NJ, USA), anti-Ly6G (#17-9668-80, Thermo Fisher Scientific, Waltham, MA, USA), annexin V (#640920, BioLegend San Diego, CA, USA), and Fixable viability dye (eFluorTM506 CYAN, # 65-0866-14, eBioscience,

(19) Specific overexpression of SIRT1 in mesenchymal stem cells rescues hematopoiesis niche in BMI1 knockout mice through promoting CXCL12 expressionInternational journal of biological sciencesJanuary 1, 2022Li J, Li X, Sun W, Zhang J, Yan Q, Wu J, Jin J, Lu R, Miao D.analysis. To detect Annexin V, CD44 and CXCR4 expression on LSK surfaces, APC-conjugated Annexin V (#640920) or CD44 (#103012) or CXCR4 (#146508) from BioLegend were added in staining panel with LSK surface markers

(20) A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell TranscriptomicsCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST.RNAscope Multiplex Fluorescent Reagent Kit v2,"Advanced Cell Diagnotics (Newark, CA)",323100 Antibodies,, AnnexinV-APC,BioLegend,640920 Lysozyme,"Diagnostic Biosystems (Pleasanton, CA)",RP028 CFTR,Cystic Fibrosis Foundation,A570 Mucin

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(1)

8/6/2018Annexin V APC for Determining Apoptosis in Cells Suzanne TomchuckWe often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the... Read Review
(1)

2/24/2017Staining T Cells With Annexin V APC Adam GuessThis reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other ... Read Review

APC Annexin V from BioLegend

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APC Annexin V from BioLegend

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