FITC Annexin V Apoptosis Detection Kit with PI from BioLegend

Product Specs
ItemFITC Annexin V Apoptosis Detection Kit with PI
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640914
Quantity100 tests
ApplicationsFlow Cytometry (FC)
ReactivityHuman, Mouse, Rat
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Antibody TypePrimary
Conjugate/Tag/LabelFITC
TargetAnnexin V

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes NephropathyInt J Mol SciDecember 18, 2023Shicong Song, Tiantian Hu, Xu Shi, Yongjie Jin, Sirui Liu, Xuehong Li, Wei Zou, Cheng Wangaccording to the manufacturer’s instructions. Briefly, the cells were stained with annexin V-FITC (Cat#640914, Biolegend, San Diego, CA, USA) for 20 min at 37 °C in annexin V binding buffer. Then, propidium iodide

(2) Determination of plastic polyester oligomers in real samples and their bioeffectsEFSA JournalDecember 4, 2023Sara Di Lonardo, Dimitra Diamantidou, Aikaterini‐Theodora Chatzitaki, Dimitris Fatouros, Georgios Theodoridislevel. Apoptosis were checked by using Fluoroisothiocyanate Annexin V Apoptosis Detection Kit (BioLegend; Cat# 640914). Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to

(3) Kaposi’s sarcoma-associated herpesvirus viral protein kinase augments cell survivalCell Death & DiseaseOctober 18, 2023Xin-Jun Wu, Zhigang Zhang, Jason P. Wong, Ricardo Rivera-Soto, Maria C. White, Aryan A. Rai, Blossom Damaniausing the Annexin V-FITC/PI staining kit according to the manufacturer’s instructions (Biolegend, #640914). Samples were processed on a MACSQuant VYB flow cytometer (Miltenyi Biotec), and data were analyzed

(4) The anti-cancer efficacy of a novel phenothiazine derivative is independent of dopamine and serotonin receptor inhibitionFrontiers in OncologyOctober 16, 2023Marion Vanneste, Anita Venzke, Soumitra Guin, Andrew J. Fuller, Andrew J. Jezewski, Sarah R. Beattie, Damian J. Krysan, Marvin J. Meyers, Michael D. HenryModFit LT software. Apoptosis was assessed with the Annexin V Apoptosis Detection kit (Biolegend cat#640914, San Diego, CA) according to the manufacturer’s instructions. Samples were processed with the Becton

(5) Glucose Oxidation-Dependent Survival of Activated B Cells Provides a Putative Novel Therapeutic Target for Lupus TreatmentiScienceSeptember 15, 2023John J. Wilson, Jian Wei, Andrea R. Daamen, John D. Sears, Elaine Bechtel, Colleen L. Mayberry, Grace A. Stafford, Lesley Bechtold, Amrie C. Grammer, Peter E. Lipsky, et al.Fixation and Permeabilization Solution,BD Biosciences,Cat#554722 FITC Annexin V Apoptosis Detection Kit with PI,BioLegend,Cat#640914 Seahorse XF Real-Time ATP Rate Assay Kit,Agilent,Cat#103592 Glucose Assay Kit,Eton Bioscience,Cat#1200032002 miRNeasy

(6) Riluzole Suppresses Growth and Enhances Response to Endocrine Therapy in ER+ Breast CancerJournal of the Endocrine SocietyAugust 28, 2023Ayodeji O Olukoya, Hillary Stires, Shaymaa Bahnassy, Sonali Persaud, Yanira Guerra, Suman Ranjit, Shihong Ma, M Idalia Cruz, Carlos Benitez, Aaron M Rozeboom, et al.quantified by flow cytometry. The PI/annexin V-FITC apoptosis detection kit was purchased from BioLegend (#640914, San Diego, CA, USA). Data are presented as mean ± SD for 3 (SUM44) or 4 (LCCTam) independent biological assays

(7) TAF1B depletion leads to apoptotic cell death by inducing nucleolar stress and activating p53-miR-101 circuit in hepatocellular carcinomaFrontiers in OncologyAugust 14, 2023Hang-fei Chen, Dan-dan Gao, Xin-qing Jiang, Hao Sheng, Qi Wu, Quan Zheng, Qiao-cheng Zhai, Lei Yuan, Ming Liu, Li-feng Xu, et al.software. Apoptosis assay Apoptotic cells were detected using the FITC Annexin V Apoptosis Detection Kit with PI (#640914, BioLegend, San Diego, CA, USA). The cells were harvested, washed with cold PBS, and re-suspended with

(8) Evaluation of cytotoxic and apoptotic effects of the extracts and phenolic compounds of Astragalus globosus Vahl and Astragalus breviflorus DCSaudi Pharmaceutical JournalAugust 1, 2023Benan Kalaycı, Nihal Şimşek Özek, Ferhunde Aysin, Hilal Özbek, Cavit Kazaz, Mehmet Önal, Zühal Güvenalpsoftware. 2.5 Flow cytometry Cell death types were determined using the Annexin V-FITC/PI (Biolegend, 640914) via flow cytometry of the cell lines. The cell lines (3 × 105 cells/2 mL per well) were seeded in six-well

(9) Clearance of defective muscle stem cells by senolytics restores myogenesis in myotonic dystrophy type 1Nature CommunicationsJuly 19, 2023Talita C. Conte, Gilberto Duran-Bishop, Zakaria Orfi, Inès Mokhtari, Alyson Deprez, Isabelle Côté, Thomas Molina, Tae-Yeon Kim, Lydia Tellier, Marie-Pier Roussel, et al.cells were identified using a detection kit (Biolegend, FITC Annexin V Apoptosis Detection Kit with PI, 640914) according to manufacturer’s specifications. After trypsinization, cells were washed twice with Cell Staining Buffer

(10) PARP1 Inhibition Halts EBV+ Lymphoma Progression by Disrupting the EBNA2/MYC AxisbioRxivJuly 12, 2023Giorgia Napoletani, Samantha S. Soldan, Toshitha Kannan, Sarah Preston-Alp, Peter Vogel, Davide Maestri, Lisa Beatrice Caruso, Andrew Kossenkov, Asher Sobotka, Paul M. Lieberman, et al.CellTiterGlow/Resazurin and analyzed on GraphPad. FITC Annexin V PI Apoptosis Detection Kit (BioLegend, Cat. No. 640914) was used to study the apoptosis induction caused by BMN-673 following manufacturer instructions. Briefly,

(11) Forkhead box protein D2 suppresses colorectal cancer by reprogramming enhancer interactionsNucleic Acids ResearchJuly 7, 2023Hyo-Min Kim, Byunghee Kang, Sohyun Park, Hyorim Park, Chan Johng Kim, Hyeonji Lee, Mijoung Yoo, Mi-Na Kweon, Sin-Hyeog Im, Tae Il Kim, et al.twice with cold 1× PBS and analyzed with the FITC Annexin V Apoptosis Detection Kit with PI (BioLegend, 640914) according to the manufacturer's instructions. The scratch assay was performed to assess the cell migration

(12) Systems-level analyses of protein-protein interaction network dysfunctions via epichaperomics identify cancer-specific mechanisms of stress adaptationNature communicationsJune 23, 2023Rodina A, Xu C, Digwal CS, Joshi S, Patel Y, Santhaseela AR, Bay S, Merugu S, Alam A, Yan P, et al.Following gentle vortexing, 8 µL of annexin-V-FITC and 8 µL of propidium iodide (Biolegend, Cat. No. 640914) were added to each sample. Cells were incubated for 25 min at room temperature in the dark and analysed

(13) Bioinspired engineering of fusogen and targeting moiety equipped nanovesiclesNature CommunicationsJune 8, 2023Lixue Wang, Guosheng Wang, Wenjun Mao, Yundi Chen, Md. Mofizur Rahman, Chuandong Zhu, Peter M. Prisinzano, Bo Kong, Jing Wang, Luke P. Lee, et al.in 100 μl of 1× binding buffer (BD Biosciences, 556454) were incubated with 50 µg/ml PI (BioLegend, 640914) overnight at 4 °C. Cell cycle analysis was performed with flow cytometry (BD Accuri C6). Data was processed

(14) SMARCB1 regulates a TFCP2L1-MYC transcriptional switch promoting renal medullary carcinoma transformation and ferroptosis resistanceNature CommunicationsMay 26, 2023Bujamin H. Vokshi, Guillaume Davidson, Nassim Tawanaie Pour Sedehi, Alexandra Helleux, Marc Rippinger, Alexandre R. Haller, Justine Gantzer, Jonathan Thouvenin, Philippe Baltzinger, Rachida Bouarich, et al.co-stained with Annexin-V-FITC and propidium iodide following manufacturer instructions (BioLegend, #640914). To assess active Caspase-3, cells were fixed and permeabilized before incubation with the FITC-conjugated

(15) Mitochondrial protein import clogging as a mechanism of diseaseeLifeMay 2, 2023Liam P Coyne, Xiaowen Wang, Jiyao Song, Ebbing de Jong, Karin Schneider, Paul T Massa, Frank A Middleton, Thomas Becker, Xin Jie ChenMix,Promega,Cat. #L2080, Commercial assay or kit,FITC Annexin V Apoptosis Detection Kit with PI,BioLegend,Cat. #640914, Commercial assay or kit,Quick-RNA Fungal/Bacterial Microprep Kit,Zymo,Cat. #R2010, Commercial assay

(16) Utilization of Dairy By-Products as a Source of Functional and Health Compounds—The Role of Ovine Colostrum and Milk Whey on Chronic Myeloid Leukemia CellsFoodsApril 23, 2023Carlotta Ceniti, Rosa Luisa Ambrosio, Jessica Bria, Anna Di Vito, Bruno Tilocca, Aniello Anastasio, Domenico Britti, Valeria Maria Morittu, Emanuela Chiarellaapoptotic cells was performed by using an FITC Annexin V Apoptosis Detection kit with PI (Biolegend #640914), as described in [27]. Briefly, 2 × 105 cells were subjected to single or repeated daily treatment

(17) Protein arginine N-methyltransferase 4 (PRMT4) contributes to lymphopenia in experimental sepsisThoraxApril 1, 2023Lai Y, Li X, Li T, Li X, Nyunoya T, Chen K, Kitsios G, Nouraie M, Zhang Y, McVerry BJ, et al.software (Tree Star, Oregon, USA). FITC Annexin V Apoptosis Detection Kit with propidium iodide (cat# 640914; BioLegend, California, USA) was employed to detect apoptotic cells in Jurkat cells. The samples were

(18) Modulation of cytotoxic amyloid fibrillation and mitochondrial damage of α-synuclein by catechols mediated conformational changesScientific ReportsMarch 31, 2023Toktam Zohoorian-Abootorabi, Ali Akbar Meratan, Saeed Jafarkhani, Vladimir Muronetz, Thomas Haertlé, Ali Akbar SabourySH-SY5Y cells were stained with the Annexin V-FITC/PI double staining apoptosis detection kit (BioLegend, 640914) at RT for 20 min, according to the manufacturer datasheet. Subsequently, the samples were analyzed

(19) Protein kinase A activation alleviates cataract formation via increased gap junction intercellular communicationiScienceMarch 17, 2023Yu Du, Yuxin Tong, Yumeng Quan, Guangyan Wang, Hongyun Cheng, Sumin Gu, Jean X. Jiangassays,Critical commercial assays,Critical commercial assays ,, Dead Cell Apoptosis Kit,Biolegend,Cat# 640914 PKA Colorimetric Activity Kit,Thermo Fisher Scientific,Cat# EIAPKA ,, Experimental models: Cell lines,Experimental models

(20) lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cellsExperimental and Therapeutic MedicineFebruary 22, 2023Shu Chen, Yaozhong Liang, Yuan Shen, Xiaoyu Wangassay To detect apoptosis, EC cells were stained with the Annexin V-FITC/PI Dual Staining kit (cat. no. #640914; Biolegend, Inc.) and then subjected to flow cytometric analysis. Briefly, cells were digested by 0.5%

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5/16/2023Useful Annexin V/PI Kit for Examining and Identifying Apoptotic Cells Robert YuanWe wanted to assess the potential of some drugs to kill cancer cells a certain way, so we used to kit to identify fully dead and apoptotic cells.

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FITC Annexin V Apoptosis Detection Kit with PI from BioLegend

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FITC Annexin V Apoptosis Detection Kit with PI from BioLegend

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