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Biomolecules
Recombinant Human IL-12 (p70) (carrier-free)
Recombinant Human IL-12 (p70) (carrier-free) from BioLegend
Be the first to write a review!
Citations:
(18)
Supplier Page
Supplier Page from
BioLegend for
Recombinant Human IL-12 (p70) (carrier-free)
Description
Recombinant Human IL-12 (p70) (carrier-free)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Recombinant Human IL-12 (p70) (carrier-free)
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
573004
Applications
Bioassay
Quantity
25 µg
Gene Name
tumor necrosis factor
NCBI Gene Aliases
CLMF, IL-12A, NFSK, NKSF, NKSF2, NKSF1, CLMF2, P35, IMD29, IMD28, IL-12B
NCBI Full Gene Name
interleukin 12B, interleukin 12A
Format
The protein was 0.22µm filtered in PBS, pH 7.2
Molecule Name
IL-12 p70
Concentration
0.20 mg/mL
Species
Human
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BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (18)
(1)
Immunoengineered MXene nanosystem for mitigation of alloantigen presentation and prevention of transplant vasculopathy
Nano Today
February 1, 2023
Weiang Yan, Alireza Rafieerad, Keshav Narayan Alagarsamy, Leena Regi Saleth, Rakesh C. Arora, Sanjiv Dhingra
recombinant human IL-2 (
589102
,
BioLegend
). To induce TH1 polarization, 10 ng/mL of recombinant human IL-12 (
573002
,
BioLegend
) was added to the culture medium. The
co-cultures
of HUVECs and
human
PBMNCs were performed
(2)
MHC class II molecules on pancreatic cancer cells indicate a potential for neo-antigen-based immunotherapy
OncoImmunology
December 31, 2022
Renato B. Baleeiro et al.
(
Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation
; #
11132D
;
Invitrogen
) and 5 ng/mL human recombinant IL-12 (#
573002
;
BioLegend
).
Human
recombinant IL-2 (#
589106
;
BioLegend
) at 30 U/mL and
IL-15
(#
570304
;
BioLegend
) at
(3)
The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation
PLoS biology
August 1, 2022
Smith KJ, Minns D, McHugh BJ, Holloway RK, O'Connor R, Williams A, Melrose L, McPherson R, Miron VE, Davidson DJ,
et al
.
cathelicidin (LL-37). Five days later, cells were washed twice and replated with 50 ng/ml rIL-12 (
Biolegend
#
573002
) again with or without LL-37. Three days later, IFN-γ production was assessed by
flow cytometry
.
In vitro
(4)
Novel Small Molecule Tyrosine Kinase 2 Pseudokinase Ligands Block Cytokine-Induced TYK2-Mediated Signaling Pathways
Frontiers in Immunology
May 20, 2022
Yu Zhou et al.
by serially-diluted test compounds, and incubated with 40 ng/mL recombinant human IL12 (
Biolegend
, #
573002
) for overnight in a humidified, 5% CO2
cell culture
incubator at 37°C. The supernatant was then collected
(5)
Alcohol Impairs Immunometabolism and Promotes Naïve T Cell Differentiation to Pro-Inflammatory Th1 CD4+ T Cells
Frontiers in Immunology
May 12, 2022
Patrick M. McTernan, Danielle E. Levitt, David A. Welsh, Liz Simon, Robert W. Siggins, Patricia E. Molina
of soluble anti-
CD28
(2.5 μg/ml; catalog #
16-0289-81
;
ThermoFisher
) and
IL-12
(25 ng/ml; catalog #
573002
;
Biolegend
, San Diego, CA) for 3 days in 37°C incubators with 5% CO2 with and without 50 mM ethanol.
(6)
CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci
SSRN Electronic Journal
March 31, 2022
Jonathan Martinez-Fabregas, Luopin Wang, Elizabeth Pohler, adeline cozzani, Stephan Wilmes, Majid Kazemian, Ignacio Moraga
StemCell Technologies
Cat#
70025
Recombinant human Interleukin-2 Novartis Cat#709421 Recombinant human Interleukin-12
Biolegend
Cat#
573002
Recombinant human Interleukin-1b R&D Systems Cat#201-LB/CF Biological Samples Chemicals, Peptides,
(7)
Fabrication of Smart Tantalum Carbide MXene Quantum Dots with Intrinsic Immunomodulatory Properties for Treatment of Allograft Vasculopathy
Advanced Functional Materials
November 10, 2021
Alireza Rafieerad, Weiang Yan, Keshav Narayan Alagarsamy, Abhay Srivastava, Niketa Sareen, Rakesh C. Arora, Sanjiv Dhingra
BioLegend). For TH1 polarization, the medium was also supplemented with 10 ng mL−1 recombinant human IL‐12 (
573002
,
BioLegend
). The cells were cultured for one week and analyzed using
flow cytometry
.
Flow Cytometry
The
(8)
Identifying cytokine signaling signatures in primary human Th-1 cells by phospho-proteomics analysis
STAR protocols
June 18, 2021
Martinez-Fabregas J, Pohler E, Moraga I.
proteins" ,, Recombinant human interleukin-2,Novartis,Cat#709421 Recombinant human interleukin-12,
BioLegend
,Cat#
573002
MSC2530818,Stratech,Cat#S8387-SEL Recombinant human hyper-interleukin-6,Expressed and purified in
(9)
Competitive binding of STATs to receptor phospho-Tyr motifs accounts for altered cytokine responses
eLife
April 19, 2021
Stephan Wilmes et al.
#709421, 20 ng/ml), anti-IL-4 antibody (10 ng/ml,
BD Biosciences
, #
554481
), IL-12 (20 ng/ml,
BioLegend
, #
573002
). After 3 days of priming, cells were expanded for another 5 days in the presence of IL-2 (20 ng/ml).
Human
(10)
CDK8 Fine-Tunes IL-6 Transcriptional Activities by Limiting STAT3 Resident Time at the Gene Loci
Cell reports
December 22, 2020
Martinez-Fabregas J, Wang L, Pohler E, Cozzani A, Wilmes S, Kazemian M, Mitra S, Moraga I.
manufacturer instructions in the presence of 20 ng/mL IL2 (Novartis #709421), 20 ng/mL IL12 (
BioLegend
, #
573002
) and 10 ng/mL anti-
IL4
(
BD Biosciences
, #
554481
). Then, cells were split into three different conditions
(11)
Paradoxical Pro-inflammatory Responses by Human Macrophages to an Amoebae Host-Adapted Legionella Effector
Cell host & microbe
April 8, 2020
Price C, Jones S, Mihelcic M, Santic M, Abu Kwaik Y.
570202
Human IL-4,
Biolegend
,Cat #
574002
Human TNF-α,
Biolegend
,Cat #
570102
Human IL-12p70,
Biolegend
,Cat #
573002
M-Per Mammalian Protein Extraction Reagent
,
Thermo Scientific
,Cat #
78505
Koningic acid,
Cayman Chemical
,Cat
(12)
Kinetics of cytokine receptor trafficking determine signaling and functional selectivity
eLife
November 27, 2019
Martinez-Fabregas J, Wilmes S, Wang L, Hafer M, Pohler E, Lokau J, Garbers C, Cozzani A, Fyfe PK, Piehler J,
et al
.
instructions in the presence of IL2 (20 ng/ml, Novartis Cat#709421), IL12 (20 ng/ml,
BioLegend
, Cat#
573002
) and anti-
IL4
(10 ng/ml,
BD Biosciences
, Cat#
554481
). Then, cells were expanded in the presence of IL2
(13)
Peptidylarginine deiminases 2 and 4 modulate innate and adaptive immune responses in TLR-7-dependent lupus
JCI insight
December 6, 2018
Liu Y, Lightfoot YL, Seto N, Carmona-Rivera C, Moore E, Goel R, O'Neil L, Mistry P, Hoffmann V, Mondal S,
et al
.
(14)
TGFβ Imprinting During Activation Promotes Natural Killer Cell Cytokine Hypersecretion
Cancers
November 5, 2018
Foltz JA, Moseman JE, Thakkar A, Chakravarti N, Lee DA.
IL-12,
IL-15
, and
IL-18
, primary
NK cells
were stimulated overnight with 10 ng/mL IL-12 (
Biolegend
,
573002
), 50 ng/mL
IL-15
(
Biolegend
,
570302
) and 50 ng/mL
IL-18
(
Biolegend
,
592102
) as described and rested
(15)
CD4 T-cells regulate angiogenesis and myogenesis
Biomaterials
September 1, 2018
Brian J. Kwee, Erica Budina, Alexander J. Najibi, David J. Mooney
the respective phenotypes: 5 μg/mL anti-IL-4 (
BioLegend
#
500815
), 10 ng/mL human IL-12 (
BioLegend
#
573002
), 50 ng/mL human IL-2 for Th1; 5 μg/mL
anti-IFN-γ
(
BioLegend
#
506513
), 25 ng/mL human IL-4 (R&D #204-IL-010),
(16)
L-Arginine Modulates T Cell Metabolism and Enhances Survival and Anti-tumor Activity
Cell
October 20, 2016
Geiger R, Rieckmann JC, Wolf T, Basso C, Feng Y, Fuhrer T, Kogadeeva M, Picotti P, Meissner F, Mann M,
et al
.
(FCCP),
Sigma
,Cat#
C2920
Antimycin,
Sigma
,Cat#
A8674
Recombinant human interleukin-2,
BD Biosciences
,Cat#
554603
Recombinant human interleukin-12,
Biolegend
,Cat#
573002
Human
recombinant interleukin-2 (
transfected
J588L cell supernatant),In house,N/A FlowCytomix basic
(17)
Broad-spectrum antibodies against self-antigens and cytokines in RAG deficiency
The Journal of clinical investigation
November 2, 2015
Walter JE, Rosen LB, Csomos K, Rosenberg JM, Mathew D, Keszei M, Ujhazi B, Chen K, Lee YN, Tirosh I,
et al
.
catalog BTCYT-204); IFN-ω (
Thermo Fisher Scientific
; catalog
BMS304
); or IL-12p70 (
BioLegend
; catalog
573004
) by
incubation
overnight at 4°C. Plates were then washed in PBS-Tween 0.05%, blocked by
incubation
with
(18)
A technique for the measurement of breakage and repair of DNA alkylated in vivo
Chemico-biological interactions
December 1, 1978
A Eastman, E Bresnick
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Recombinant Human IL-12 (p70) (carrier-free) from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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