BglII from New England Biolabs

Product Specs
ItemBglII
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0144L
Concentration50 u/µL
Quantity10000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

A/GATCT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Lack of evidence for ribosomal frameshifting in ATP7B mRNA decodingMolecular CellOctober 6, 2022Gary Loughran, Alla D. Fedorova, Yousuf A. Khan, John F. Atkins, Pavel V. Baranov2000,Invitrogen,11668-027 Optimem,Invitrogen,51985-026 L-glutamine,Sigma,G7513 Pen/Strep,Sigma,P4333 FBS,Sigma,F7524 DMEM,Sigma,D6429 Restriction enzyme XhoI,NEB,R0146L Restriction enzyme BglII,NEB,R0144L Restriction enzyme BamHI-HF,NEB,R3136L Phusion® High-Fidelity DNA Polymerase,NEB,M0530L T4 DNA ligase,NEB,M0202S Passive

(2) LncRNA HOTAIR sponges miR-301a-3p to promote glioblastoma proliferation and invasion through upregulating FOSL1Cellular SignallingJune 1, 2022Shanchun Guo, Pendelton King, Emily Liang, Alyssa A. Guo, Mingli LiuMluI [New England Biolabs Inc. (NEB), Ipswitch, MA, NEB, cat. no. R0198L] and BglII (NEB, cat. no. R0144L) sites. Primers for amplification are listed in Table 1. For mutated construct, mutant FOSL1 or mut-FOSL1,

(3) rigrag: high-resolution mapping of genic targeting preferences during HIV-1 integration in vitro and in vivoNucleic Acids ResearchJuly 21, 2021Gregory J Bedwell, Sooin Jang, Wen Li, Parmit K Singh, Alan N Engelmanendonucleases MseI (New England Biolabs, Ipswich, MA; catalogue number R0525L) and BglII (catalogue number R0144L) were used to fragment genomic DNA for integration site sequencing. Sequences of DNA oligonucleotides

(4) Three New Integration Vectors and Fluorescent Proteins for Use in the Opportunistic Human Pathogen Streptococcus pneumoniaeGenes (Basel)May 22, 2019Lance E. Keller, Anne-StÃ©phanie Rueff, Jun Kurushima, Jan-Willem Veening

(5) Capsid-CPSF6 Interaction Licenses Nuclear HIV-1 Trafficking to Sites of Viral DNA IntegrationCell Host & MicrobeSeptember 1, 2018Vasudevan Achuthan, Jill M. Perreira, Gregory A. Sowd, Maritza Puray-Chavez, William M. McDougall, Adriana Paulucci-Holthauzen, Xiaolin Wu, Hind J. Fadel, Eric M. Poeschla, Asha S. Multani, et al.#R0199L SpeI,New England Biolabs,Cat #R0133L MseI,New England Biolabs,Cat #R0525L BglII,New England Biolabs,Cat #R0144L Gibson assembly master mix,New England Biolabs,Cat #E2611L Critical Commercial Assays,Critical Commercial

(6) Luminal lncRNAs Regulation by ERα-Controlled Enhancers in a Ligand-Independent Manner in Breast Cancer CellsInternational Journal of Molecular SciencesFebruary 16, 2018Valentina Miano, Giulio Ferrero, Valentina Rosti, Eleonora Manitta, Jamal Elhasnaoui, Giulia Basile, Michele De BortoliDNA. DNA digestion will be done by adding a total of 450 U of restriction enzyme (BglII and NsiI, NEB R0144L and R0127L respectively) overnight at 37 ◦ C while shaking at 300 rpm. A 4.5 µL aliquot was taken by

(7) Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clustersScience AdvancesJune 21, 2017Kobe C. Yuen, Brian D. Slaughter, Jennifer L. Gertonand incubated at 37°C for 3 hours while shaking at 900 rpm. After quenching the SDS, 200 U of Bgl II (R0144L, NEB) was added and incubated at 37°C for 4 hours while shaking at 900 rpm. Additional 200 U of Bgl

(8) Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 GenerationPLoS ONESeptember 25, 2014Rolf B. Edvardsen, Sven Leininger, Lene Kleppe, Kai Ove Skaftnesmo, Anna Wargelius

(9) Fusion of fluorescent protein to puromycin N-acetyltransferase is useful in Drosophila Schneider S2 cells expressing heterologous proteinsCytotechnologyMarch 1, 2013Kotomi Nagahashi, Kazuo Umemura, Naohiro Kanayama, Takayuki Iwaki

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10/2/2015High Quality Restriction Enzyme for Cloning Prabhakaran KumarI used this BglII restriction enzyme along with EcoRV for the cloning of my gene of interest into a plasmid vector. Subsequently, I also used this enzyme for colony screening. This enzyme completely ... Read Review

BglII

BglII from New England Biolabs

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New England Biolabs

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BglII from New England Biolabs

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