7-AAD Viability Staining Solution from BioLegend

Product Specs
Item7-AAD Viability Staining Solution
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number420404
ApplicationsFlow Cytometry (FC)
Quantity500 tests
FormatPBS, pH 7.2 with 0.09% (w/v) sodium azide
Target7-AAD
Concentration50.00 µg/mL

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Bruton's tyrosine kinase-bearing B cells and microglia in neuromyelitis optica spectrum disorderJ NeuroinflammationDecember 21, 2023Ye Liu, Zhenning Huang, Tian-Xiang Zhang, Bin Han, Guili Yang, Dongmei Jia, Li Yang, Qiang Liu, Alexander Y. L. Lau, Friedemann Paul, et al.solution (BioLegend, 422302) and thereafter, incubated with 7-AAD Viability Staining Solution (BioLegend, 420403), CD3 (BioLegend, clone OKT3), CD14 (BioLegend, clone 63D3) and CD16 (BioLegend, clone 3G8). B cells

(2) Immune checkpoints are predominantly co-expressed by clonally expanded CD4+FoxP3+ intratumoral T-cells in primary human cancersJ Exp Clin Cancer ResDecember 6, 2023Delphine Bredel, Edi Tihic, Séverine Mouraud, François-Xavier Danlos, Sandrine Susini, Marine Aglave, Alexia Alfaro, Chifaou Mohamed-Djalim, Mathieu Rouanne, Héloise Halse, et al.anti-CD45-FITC mAbs (Biolegend, 304038), washed, and resuspended in PBS 0,04% BSA. 7-AAD (Biolegend, 420404) was added to the cells prior to sorting. At least 50,000 live CD45 positive cells were sorted in PBS

(3) Unbiased assessment of genome integrity and purging of adverse outcomes at the target locus upon editing of CD4+ T‐cells for the treatment of Hyper IgM1The EMBO JournalDecember 1, 2023Daniele Canarutto, Claudia Asperti, Valentina Vavassori, Simona Porcellini, Elisabetta Rovelli, Marianna Paulis, Samuele Ferrari, Angelica Varesi, Martina Fiumara, Aurelien Jacob, et al.Pharmingen,C40‐1457,560326,1:25 CD154,PE,Invitrogen,24‐31,12‐548‐42,5:100 CD154,PE,Biolegend,24‐31,310806,1:50 7‐AAD Viability Staining Solution,7‐AAD,Biolegend,‐,420404,1:100

(4) Humanized single-domain antibody targeting HER2 enhances function of chimeric antigen receptor T cellsFrontiers in ImmunologyNovember 7, 2023Rui Zheng, Yuankun Chen, Yiting Zhang, Sixin Liang, Xiaojuan Zhao, Yiyi Wang, Pengju Wang, Ruotong Meng, Angang Yang, Bo YanV (1:20, Cat# 640951, BioLegend) and its binding buffer (Cat# 422201, BioLegend), 7-AAD (1:20, Cat# 420404, BioLegend), Granzyme B (1:20, Cat# 372206, BioLegend) were used. A total of 1 × 106 cells per group

(5) Loss of Cadherin-11 in pancreatic ductal adenocarcinoma alters tumor-immune microenvironmentFrontiers in OncologyOctober 26, 2023Aimy Sebastian, Kelly A. Martin, Ivana Peran, Nicholas R. Hum, Nicole F. Leon, Beheshta Amiri, Stephen P. Wilson, Matthew A. Coleman, Elizabeth K. Wheeler, Stephen W. Byers, et al.minutes in the dark. Cells were resuspended in 7-AAD Viability Staining Solution (BioLegend, Catalog #420404) and analyzed on a BD FACSMelody cytometer, sorting CD45+/7-AAD- and CD45+/7-AAD- populations for scRNA-seq

(6) Single-cell profiling coupled with lineage analysis reveals vagal and sacral neural crest contributions to the developing enteric nervous systemeLifeOctober 25, 2023Jessica Jacobs-Li, Weiyi Tang, Can Li, Marianne E BronnerThe cells were resuspended in HBSS-BSA, supplemented 7-AAD Viability Staining Solution (BioLegend # 420404, 500 TESTS), and sorted for YFP+, viable single cells using Sony SY3200 cell sorter at the Caltech Flow Cytometry

(7) TIM-3 Expression and M2 Polarization of Macrophages in the TGFβ-Activated Tumor Microenvironment in Colorectal CancerCancers (Basel)October 11, 2023Masanori Katagata, Hirokazu Okayama, Shotaro Nakajima, Katsuharu Saito, Takahiro Sato, Mei Sakuma, Satoshi Fukai, Eisei Endo, Wataru Sakamoto, Motonobu Saito, et al.Pacific Blue-conjugated anti-human CD86 mAb (305423, BioLegend), 7-AAD Viability Staining Solution (420404, BioLegend), PE-conjugated anti-human TIM-3 mAb (345005, BioLegend) and PE-conjugated isotype control

(8) Unravelling human hematopoietic progenitor cell diversity through association with intrinsic regulatory factorsbioRxivSeptember 7, 2023Patricia Favaro, David R. Glass, Luciene Borges, Reema Baskar, Warren Reynolds, Daniel Ho, Trevor Bruce, Dmitry Tebaykin, Vanessa M. Scanlon, Ilya Shestopalov, et al.EQ, Four Element",Standard BioTools Inc.,Cat#201078 7-AAD Viability Staining Solution,BioLegend,Cat#420404 ,, Deposited Data,Deposited Data,Deposited Data ,, Mass cytometry batch-corrected fcs files,This

(9) Discovery and multimerization of cross-reactive single-domain antibodies against SARS-like viruses to enhance potency and address emerging SARS-CoV-2 variantsSci RepAugust 22, 2023Scott A. Hollingsworth, Cameron L. Noland, Karin Vroom, Anasuya Saha, Miranda Sam, Qinshan Gao, Haihong Zhou, David U. Grandy, Sujata Singh, Zhiyun Wen, et al.goat anti-llama IgG (H + L) FITC conjugate (Thermo Cat# A16061), 7-AAD live/dead cell stain (Biolegend, Cat# 420404), and streptavidin BV421 (Biolegend Cat# 405226). Live B-cells, positive for biotinylated antigen and

(10) Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscleiScienceAugust 18, 2023Uapinyoying P, Hogarth M, Battacharya S, Mázala DAG, Panchapakesan K, Bönnemann CG, Jaiswal JK.DAPI,Thermo Fisher,P36935 Wheatgerm Agglutinin (1:1000),Thermo Fisher,W6748 7AAD (1:200),BioLegend,420404 ,, Deposited data,Deposited data,Deposited data ,, scRNA-seq data of WT and BLA/J FAPs generated

(11) Fluorinated amphiphilic Poly(β-Amino ester) nanoparticle for highly efficient and specific delivery of nucleic acids to the Lung capillary endotheliumBioact MaterAugust 7, 2023Zicheng Deng, Wen Gao, Fatemeh Kohram, Enhong Li, Tanya V. Kalin, Donglu Shi, Vladimir V. Kalinichenkowith PerCP-Cy5.5 (Supplemental table s1). Dead cells were stained with 7-AAD Viability Dye (BioLegend, 420404). CD31+ CD45− CD326- CD140a-cell population was identified as endothelial cells. CD45+ CD31− CD326-

(12) In vitro differentiated human CD4+ T cells produce hepatocyte growth factorFrontiers in ImmunologyJuly 13, 2023Shayne Lavondua Ford, Terkild Brink Buus, Claudia Nastasi, Carsten Geisler, Charlotte Menné Bonefeld, Niels Ødum, Anders WoetmannGolgiStop for cytokine staining (BD, P/N 554724). Viability was assessed using either 7-AAD (BioLegend, P/N 420403) or fixable viability dye eFluor780 (Invitrogen, P/N 65-0865-14). All antibodies used were from BD Biosciences:

(13) Distinct bidirectional regulation of LFA1 and α4β7 by Rap1 and integrin adaptors in T cells under shear flowCell reportsJune 27, 2023Kamioka Y, Ueda Y, Kondo N, Tokuhiro K, Ikeda Y, Bergmeier W, Kinashi T.metabisulfite,Sigma,Cat# 161519 GST-RalGDS-RBD,Ueda et al.25,N/A 7-AAD Viability Staining Solution,Biolegend,Cat# 420404 ATTO647N-NHS-ester,ATTO-TEC,Cat# AD647N-31 "1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl)

(14) Sex-Based Disparities in Leukocyte Migration and Activation in Response to Inhalation Lung Injury: Role of SDF-1/CXCR4 SignalingCellsJune 26, 2023Tanima Chatterjee, Terry L. Lewis, Itika Arora, Anastasiia E. Gryshyna, Lilly Underwood, Juan Xavier Masjoan Juncos, Saurabh Aggarwalanti-CXCR4PE(Cat # 153806, BioLegend, San Diego, CA, USA) in combination with the viability dye, 7AAD (Cat # 420403, BioLegend). Cells were also incubated with the control isotype (Cat # 400507, BioLegend) corresponding

(15) Pentoxifylline Inhibits TNF-α/TGF-β1-Induced Epithelial-Mesenchymal Transition via Suppressing the NF-κB Pathway and SERPINE1 Expression in CaSki CellsInternational Journal of Molecular SciencesJune 24, 2023Luis Arturo Palafox-Mariscal, Pablo Cesar Ortiz-Lazareno, Luis Felipe Jave-Suárez, Adriana Aguilar-Lemarroy, María Martha Villaseñor-García, José Roberto Cruz-Lozano, Karen Lilith González-Martínez, Aníbal Samael Méndez-Clemente, Alejandro Bravo-Cuellar, Georgina Hernández-FloresGibco™; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and the cells were stained with 7AAD (cat: 420403 BioLegend San Diego, CA, USA) for viability detection. Finally, 10,000 events were acquired through

(16) Peripheral T cell profiling reveals downregulated exhaustion marker and increased diversity in lymphedema post-lymphatic venous anastomosisiScienceJune 16, 2023Hirofumi Imai, Takakazu Kawase, Shuhei Yoshida, Toshiro Mese, Solji Roh, Asuka Fujita, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Atsushi Takazawa, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, 7-Aminoactinomycin D,Biolegend,Cat#420403 Zombie-NIR,Biolegend,Cat#423105 RNase H,Invitrogen,Cat#18021-071 T4 DNA polymerase,Invitrogen,Cat#18005-025 ,, Critical

(17) Comprehensive epigenomic profiling reveals the extent of disease-specific chromatin states and informs target discovery in ankylosing spondylitisCell GenomicsJune 14, 2023Andrew C. Brown, Carla J. Cohen, Olga Mielczarek, Gabriele Migliorini, Félicie Costantino, Alice Allcock, Connor Davidson, Katherine S. Elliott, Hai Fang, Alicia Lledó Lara, et al.Staining Buffer,ThermoFisher Scientific,Cat# 00-4222-26 7-AAD Viability Staining Solution,BioLegend,Cat# 420403 eBioScience™ 1-Step Fix/Lyse Solution,ThermoFisher Scientific,Cat# 00-5333-54 "PBS, pH 7.4",Gibco,Cat#10010031 BSA,New England Biolabs

(18) Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo modelsMolecular Therapy - Nucleic AcidsJune 13, 2023Hadi Bayat, Mohammad Hossein Pourgholami, Saeid Rahmani, Safura Pournajaf, Seyed Javad Mowlaconditions using flow cytometry. The samples were stained with 7-AAD (1 μL/106 cells; Biolegend; Cat. #420403) to identify dead cells. Finally, cell proliferation was evaluated using the BD FACSCalibur Flow Cytometry

(19) Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacyMolecular Therapy - Nucleic AcidsJune 13, 2023Callum McKenzie, Mohamed El-Kholy, Farhaan Parekh, Mathew Robson, Katarina Lamb, Christopher Allen, James Sillibourne, Shaun Cordoba, Simon Thomas, Martin PuleD8537) or cell staining buffer (BioLegend, 420201). Cell viability dyes used were 7-AAD (BioLegend, 420404) or Sytox blue (Thermo Fisher Scientific, S34857). To detect TCF-1 expression, cells were first surface

(20) Establishment and characterization of adult human gastric epithelial progenitor‐like cell linesCell ProliferationJune 1, 2023Yuan Gao, Ji Dong, Shuyue Qi, Xin Zhou, Xinglong Wu, Wendong Wang, Lu Wen, Wei Fu, Fuchou Tangcells were dissociated into single‐cell suspensions and stained with 7‐AAD viability staining solution (420403, BioLegend) for fluorescence‐activated cell sorting (FACS; BD FACS ARIA SORP, BD Biosciences) on Days

Write a Review

(1)

4/17/2023Easy to Use Viability Staining Solution for Live and Dead Cell Detection Soheil Tavakolpour7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis.
(1)

3/24/2023Very Good 7AAD Viability Staining for Sorting Im Hong SunOur lab is interested in understanding mechanism of peripheral tolerance. We are also interested in characterizing the immune populations in secondary lymphoid organs under different knockout ... Read Review
(1)

7/7/2022Easy to Use 7AAD Viability Stain Matthew RatsepI use this reagent to identify live/dead cells in flow cytometry experiments. The reagent is easy to use and gives good results. The only issue is that the emission spectrum is right in the middle of ... Read Review
(1)

3/13/2019Staining Human T Cells with 7-AAD Adam GuessThis reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ... Read Review
(1)

8/3/20187AAD Works Well to Determine Viability by Flow Cytometry Suzanne TomchuckIn order to examine viability or apoptosis of cells when treating with various compounds, we use 7AAD (with Annexin V if determining apoptosis) and viability is determined by flow cytometry. 7AAD ... Read Review
(1)

10/29/2015Cell Viability Stain Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

7-AAD Viability Staining Solution from BioLegend

Description

BioLegend

Citations (417)

Reviews

7-AAD Viability Staining Solution from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map