Champion pET101 Directional TOPO Expression Kit with BL21 Star (DE3) One Shot Chemically Competent E. coli from Life Technologies

Champion pET101 Directional TOPO Expression Kit with BL21 Star (DE3) One Shot Chemically Competent E. coli from Life Technologies

Description

The Champion™ pET Expression System yields the highest-level protein production in E. coli. During expression, your protein of interest can reach levels greater than 50 percent of total cellular protein. Based on T7 expression vectors originally developed by Studier and colleagues (1-3), high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coli RNA polymerase and is dedicated to the transcription of your gene of interest. Protein production is further enhanced in the system by the expression strain BL21 Star™ E. coli, which significantly improves the stability of mRNA transcripts and increases protein expression up to ten-fold. Simplified, efficient Directional TOPO® cloning allows you toquickly enter a Champion™ pET Expression vector. These kits feature linearized, topoisomerase I-activated Champion™ pET expression vectors for five-minute directional cloning. Directional TOPO® Cloning technology facilitates gene

Specifications

  • Detailed Specifications
  • ItemChampion pET101 Directional TOPO Expression Kit with BL21 Star (DE3) One Shot Chemically Competent E. coli
  • CompanyLife Technologies
  • Price Pricing InfoGet Quote
  • Catalog NumberK10101
  • Quantity20 reactions
  • Vector NamepET101/D-TOPO, pET101/D /LacZ
  • ApplicationsBacterial Expression:Protein Expression:Proteins, Expression, Isolation & Analysis:T7 Bacterial Expression
  • Conjugate/Tag/LabelC-terminal V5 epitope tag and polyhistidine region.
  • PromoterT7 and lacO
  • ResistanceAmpicillin (AmpR)
  • Selectable MarkerNone
  • TypePlasmid
  • HostBacteria
  • InducerIPTG
  • Domain/Region/TerminusC-terminal
Life Technologies
Life Technologies
5791 Van Allen Way
Carlsbad, CA 92008
United States
Phone: (760) 603-7200

Citations