TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays are a valuable method for detecting DNA fragmentation, which is a hallmark of apoptosis, or programmed cell death. In a TUNEL assay, an enzyme known as terminal deoxynucleotidyl transferase (TdT) identifies nicks, or points of fragmentation...
The carboxylate ion of pyruvic acid, pyruvate, is a central molecule in various intermediary metabolic pathways. Pyruvate is produced from glucose through glycolysis, supplies energy to cells via metabolism through the...
G protein-coupled receptors (GPCRs), integrins, ion channels and virtually every other type of membrane protein are prime research targets. As key elements in...
If you haven't explored new technologies in gene expression in the past couple of years, it may be time to upgrade your expression vector.
Cellular models of Parkinson’s Disease are useful for testing the efficacy of intrabodies (intracellular antibodies) against the pathological effects of alpha–synuclein (protein molecule implicated in the disease) and its mutants, when transiently...
Caspases are cysteine-dependent, aspartate-specific proteases, which play essential roles in apoptosis and are also required in the immune system for the maturation of cytokines (such as IL-1 and IL-18). They exist as latent...
Measuring necrosis in eukaryotic cells is a common procedure and can be performed in a variety of ways. We work with bacterial pathogens known to have deleterious effects on mammalian cells and we typically study cell death of various cell lines including...
The Biovision CaspGLOW Red Active Caspase Staining Kit is a complete set of reagents for providing an easy, fast and versatile assay to detect cells with activated caspases. The assay exploits the irreversible binding between activated caspase and a general caspase inhibitor (VAD-FMK) conjugated to sulf-rhodamine (Red-VAD-FMK) as a fluorescent marker of activity...
The Caspase-3 Fluorometric Protease Assay Kit is a great tool for determining whether Caspase-3 is activated in a given experiment. Caspase-3, sometimes called the “executioner caspase”, serves as a positive indicator of apoptosis...
The Caspase Fluorometric Substrate Set from BioVision is a reagent set designed to detect specific caspases by their enzymatic activities. The set includes reagents to screen for 7 members of the caspase family described so far (including caspase-1, -2, -3, -5, -6, -8 and -9)...