Today’s mass spectrometers are faster, more sophisticated and more sensitive than ever. Yet if the molecules that are fed into these machines aren’t charged, the machine is as blind as a bat. That’s because mass spectrometers don’t actually measure a molecule’s mass, but rather its mass-to-charge ratio (m/z). Given a molecule’s m/z value...
In this video, Dr. Patrick Luedtke, Director of the Public Health Laboratories, discusses how the Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument from Life Technologies has helped his laboratory get real-time, actionable test results for local health departments and hospital communities, which was particularly important during their response to the H1N1 influenza pandemic.
MicroRNAs are small endogenous RNAs that play a crucial role in translational repression of mRNA transcripts. Over the past decade, miRNA studies have gained high significance due to the important role they play in cellular processes. miRNA are...
Alexander Hoischen, the first author of the Nature Genetics publication, “De novo mutations of SETBP1 cause Schinzel-Giedion syndrome” describes how exome sequencing and the SOLiD™ System are being used in their laboratory in Nijmegan, The Netherlands to identify and characterize potentially causative SNPs in research of rare genetic disorders.
A quick clean-up of any large scale transcription reaction can be very laborious, time consuming and a multi-step protocol is often needed. In addition, great flexibility in regards to downstream applications is not easy...
This seminar presents SoftGenetics® NextGENe® Software, a genetic analysis tool for biologists. Demonstration of methods for structural variant discovery and visualization of results and reports using SOLiD™ System data will be shown.
This seminar is part of a continuing series of presentations aimed at showcasing the wealth of data analysis solutions available for the SOLiD™ System platform. During this seminar, you will see an introduction to the InteRNA Genomics miR-Intess™ platform, a computational analysis pipeline for digital gene expression (DGE) profiling of small RNAs with the SOLiD™ System.
Taq DNA Polymerase, an enzyme that catalyzes the incorporation of dNTPs into DNA, is one of the most commonly used polymerases in molecular biology. Though it lacks proofreading ability, and other polymerases have since been...
Occasionally, one is faced with the prospect of performing RT-PCR simultaneously on numerous samples. In my case, this occurred following the retroviral transfection of cultured lung cancer cells with an shRNA targeted against a specific transcript. Since the entire...
Nicola Oldroyd, a senior forensic scientist at Applied Boisystems, discusses the development of the AmpFlSTR® NGM™ PCR Amplification Kit.
Mike Lelivelt, Ph.D discusses the analysis of SOLiD™ System RNA-Seq data in Partek Genomics Suite.
Groove anywhere with the Veriti™ Thermal Cycler! The Veriti™ Thermal Cycler gives you peace of mind by providing you with reliability and proven performance you expect from Applied Biosystems. And with VeritiLink™ Remote Management Software, you can receive thermal cycler run updates on your PDA or phone at the club, the beach, virtually anywhere!
The SOLiD™ Whole Transcriptome Analysis Kit provides a highly sensitive assay that can detect known and novel transcripts, subtle changes in gene expression, and allele-specific expression and enabling scientists to derive biological insight from the transcriptome using the SOLiD™ System.
The TaqMan® MicroRNA Reverse Transcription Kit is specifically designed to generate microRNA specific cDNA, for use with the TaqMan® MicroRNA Assays. These assays are an invaluable tool for...
The SOLiD™ System delivers on the promise of single-cell expression analysis, offering exciting possibilities in important areas such as stem cell research, cancer biology, and developmental biology. In this online seminar, Dr. Kai Lao will share data from his recent publication on whole transcriptome analysis using single cells.