Chromatin regulators (CRs) control chromatin structure, yet the rules governing these processes remain obscure. Specific CR combinations, co-bind at genomic locations of coherent functions. The presentation will detail a systematic approach to infer the function of CRs by mapping genome-wide CR-binding, and present an approach for validating antibodies for genome-wide ChIP studies. Recommendations for characterizing ChIP-specific antibodies will be presented.
The MILLIPLEX® MAP EpiQuant™ assay, using the Luminex® xMAP® technology, is a quantitative, multiplexed immunoassay that simultaneously measures both total protein abundance and site-specific phosphorylation at the peptide level with picomolar sensitivity. The EpiQuant™ technology uses antibodies against defined protein sequences to capture peptides resulting from protein linearization and digestion in a single cell lysate sample, similar to fragments generated using mass spectroscopy. These assays require substantially less sample than traditional cell signaling immunoassays.
Dr. Christman’s laboratory has recently developed a variety of injectable ECM derived materials that self-assemble to form porous, nanofibrous scaffolds once injected in vivo or brought to physiological temperature. These scaffolds are showing promise in a variety of tissues including the myocardium, skeletal muscle, and adipose tissue.
Angiogenesis is not only a prerequisite for tumor growth, but is also a major factor affecting the metastatic spread of malignant cells. This presentation will highlight the role of angiogenesis in tumor growth and metastasis and introduce various investigative tools developed by EMD Millipore to block angiogenesis.
Speaker: Chandra Mohan, Ph.D., EMD Millipore
Date: May 24, 2012
Time: 9:30 AM PST
TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays are a valuable method for detecting DNA fragmentation, which is a hallmark of apoptosis, or programmed cell death. In a TUNEL assay, an enzyme known as terminal deoxynucleotidyl transferase (TdT) identifies nicks, or points of fragmentation...
Good staining of M-I mouse oocytes.
Not long ago, you had to wait your turn to use a FACS machine in a core facility—now you can have your own cell sorting flow cytometer in your office. Today’s sophisticated technology has made cell analysis better than ever. If you are searching for a flow cytometry system, here are some considerations and examples of the features available...
Perform multiple biomarker immunoassays using EMD Millipore’s complete, versatile workflow solution. With analytically validated MILLIPLEX® MAP assay kits, using Luminex® instruments, and MILLIPLEX Analyst software, measure one analyte per sample or fifty—in the broadest research areas including metabolism, immunology, cancer, cell signaling, cellular metabolism, neuroscience and toxicity—using preconfigured or customized panels.
Mycoplasmas are common bacterial contaminants of cell
cultures and represent a risk for products isolated from such
cultures. Myoplasmas in infected cell cultures can alter cellular
processes such as cell growth rate, cell transformation, or
morphology. In many cases contamination remains undetected
because imperceptible change in pH, culture turbidity or visibility of organisms in a microscope are observed. Mycoplasmas
grow slowly – the colonies may take up to 3 weeks to
develop – which makes traditional culture methods unacceptable
for rapid testing. The MMB Mycoplasma PCR Kit
offers a new approach for easy, fast and reliable PCR detection
followed by convenient gel electrophoresis.
If you've ever worked with cell cultures, you probably know the agony of manual cell counting. It's a fact of life in the cell culture hood: Before you can run an experiment, you need to know...
I am working as a Senior Research Fellow in the Department of Biophysics, Panjab University, Chandigarh, India. I had an opportunity to use and analyze the Caspase 9 Colorimetric Activity Assay Kit from Millipore (Chemicon International). As part of my work, I was in search of a convenient and efficient protocol for...
Flow cytometry has become more accessible to the life science community in recent years thanks to the development of smaller, more sophisticated instruments coupled with their relative affordability. One such...
Any researcher that works with proteins knows how tedious and time consuming running Western blots can be. Although the reagents involved have evolved over time, the basic protocol has remained the same. The Snap I.D. from Millipore has revolutionized...
Without ion channels, we would cease to move, think, or breathe—hence their involvement in many diseases and their attractiveness as drug targets. Indeed, recent activities in the development of drugs that target ion channels have increased our understanding of...
Cell adhesion assays measure adhesion between cells or between a cell and a surface or extracellular matrix. The most basic assay might involve plating cells onto a surface, washing the cells after time has passed, and observing the number...