In vivo, we analyzed regenerating skeletal muscle, otherwise desmin expression was very low; in vitro desmin is normally expressed by myogenic cell lines / primary cultures. Our results were quite reproducible but we had to load large amounts of protein when using whole tissue extracts.
I observed good staining in human colon epithelium cells and in smooth muscle cells (longitudinal and circular).
Emma Lundberg discusses the creation of an antibody-based subcellular protein atlas.
One of the most rapidly evolving scientific fronts, Stem Cell Reprogramming holds great promise as a tool for discovery in both disease and basic biology.
Knocking out a gene from an animal’s genetic blueprint is a powerful tool—one that has certainly seen improvements since its relatively recent inception. Indeed, the method of removing (or otherwise taking out of commission) a gene from...
Within each human genome is a record of that individual's genetic past, and a glimpse into their future. Yet it is only a glimpse. A few high-profile disease loci notwithstanding, most human traits are not written solely in triplets of As, Cs, Gs, and Ts; other factors have a say as well. One of those factors...
Quantitative real-time PCR (qPCR) is fast replacing conventional reverse transcriptase PCR in many molecular biology labs, increasingly reflected by high quality journals adjusting their requirements to only qPCR for gene expression data. qPCR relies on...
Dr. Miguel Esteban from the Chinese Academy of Sciences presents data for efficient generation of human induced pluripotent stem cells from multiple tissues.
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Molecular weight markers for gel filtration are essential components for standardization of the column and for size prediction of an unknown sample. In gel filtration, the samples are run through a gel matrix and are separated based...
Gene expression studies have deepened our understanding of important cellular functions. However, they often assume that cells of a particular type are homogeneous – and this is not necessarily the case. “Just like people, even genetically identical cells...
The Stemgent Lentivirus Reprogramming product line offers Dox-inducible or constitutive expression of factors
in polycistronic or individual formats for reprogramming mouse or human cells. We will review how each product is
validated and how to select the right product for your applications. We will also demonstrate iPS colony morphology
changes during reprogramming and identify which colonies to pick for expansion.
Since Yamanka et al first described generation of iPS cells in 2006 there have been many modifications to the
reprogramming method to improve efficiency and safety for clinical applications. We will review important
milestones since the method was first published and walk through the steps of a reprogramming experiment.
The past few years has witnessed the development of new technological platforms enabling the creation of genome-scale RNAi libraries and methodologies for their use in functional genomic research. The ultimate goal is to produce genome-wide mammalian RNAi libraries that can achieve stable and specific gene knockdown in a wide variety of cell types, and to develop the requisite tools for their implementation in high-throughput screening assays.
For patients presenting with disseminated prostate cancer, the tumor is typically dependent on androgen for growth and therefore responsive to therapies that take advantage of surgical and/or pharmacological depletion of circulating androgens. However, this type of therapeutic success is often temporary; the disease almost invariably recurs as a metastatic and androgen-independent tumor.